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Image Search Results
Journal:
Article Title: Activation of m-Calpain (Calpain II) by Epidermal Growth Factor Is Limited by Protein Kinase A Phosphorylation of m-Calpain
doi: 10.1128/MCB.22.8.2716-2727.2002
Figure Lengend Snippet: Effects of CPT-cAMPS and PKA inhibitors on EGF-induced cell migration. NR6WT cells were grown to confluence and made quiescent for 24 h in MEMα with 0.5% dialyzed fetal calf serum before treatment or not with EGF (10 nM), CPT-cAMPS (1 μM), Rp-8Br-cAMPS and/or Rp-8Br-cGMPS (5 μM) (A), and H-89 (at indicated concentrations) (B). The datum for nontreated control cells is labeled as nTx. Basal and EGF-induced cell migrations were assessed as the ability of the cells to move into an acellular area after 24 h of EGF treatment. The data are shown as the ratios to the 10 nM EGF-induced cell migration activity. The data are the means ± SEM of at least three independent studies, each performed in triplicate. Statistical analysis was performed by Student's t test. n.s., not significant.
Article Snippet: Cells were treated or not with CPT-cAMPS (1 μM) and/or
Techniques: Migration, Labeling, Activity Assay
Journal:
Article Title: Activation of m-Calpain (Calpain II) by Epidermal Growth Factor Is Limited by Protein Kinase A Phosphorylation of m-Calpain
doi: 10.1128/MCB.22.8.2716-2727.2002
Figure Lengend Snippet: EGF-induced calpain activation. NR6WT cells were plated on tissue culture chamber slides (Nunc) and made quiescent for 24 h in MEMα with 0.5% dialyzed fetal calf serum. Cells were treated or not with CPT-cAMPS (1 μM), Rp-8Br-cAMPS (5 μM), and/or Rp-8Br-cGMPS (5 μM) 30 min prior to EGF (10 nM) treatment in the presence of BOC-LM-CMAC (Molecular Probes). Then cells were treated or not with EGF (10 nM) for 5 min. Calpain activation was assessed by fluorescence microscopy. The fluorescence indicates calpain activity. The panel for nontreated control cells is labeled as nTx. The pictures shown are representative of n = 9.
Article Snippet: Cells were treated or not with CPT-cAMPS (1 μM) and/or
Techniques: Activation Assay, Fluorescence, Microscopy, Activity Assay, Labeling